Evaluation of Nutritional Value for Four Kinds of Wild Plants in Northern Sector of Nile Delta, Egypt

Wild plants play an essential role in non-traditional fodder producing plants. The aim of the study is to evaluate nutritional composition of wild plants. These plants are Calligonum polygonoides, Cakile maritima, Senecio glaucus (Psammophytes) and Zygophullum album (halophyte) naturally growing in Deltaic Mediterranean coastal desert of Egypt. These plants are rich with their nutritive values and their water requirements are low. The protein content of the investigated wild species ranges from 5.90% to 19.63% and the fat content ranges from 0.75% to 1.93%. The amount of nitrogen and phosphorus are higher in all plants followed by potassium, calcium, magnesium and sodium. On the other hand, the microelements levels in the studied wild plants exist as Fe > Mn > Zn > Cu > Ni > Pb > Co > Cd. Finally, the phytochemical analysis of the studied wild plants indicates that the four wild plants are important and there is a need to develop new techniques for cultivation as well as processing of such plants for future use.

• Proximate Composition
• Minerals
• Antioxidant
• Wild Species
• Costal Desert

1. Introduction

The population density of the Egyptian desert is generally, low, and hundreds of square kilometers may be devoid of human habitation. The reverse is true in the River Nile region which covers only less than 4% of Egypt’s lands. In the same time it has very dense population that is expected to be hundred millions by 2020. Therefore, areas of desert vacant to-day should be habituated. Halophytic plants differ in their nutritive values from one species to another [1] .

The mineral nutrition is an important aspect and it plays an essential role in organisms’ life for healthy growth. Such type of mineral is easily available in wild edible plants [2] . The World Health Organization (1982) had reported that chronic under-nutrition affects some 200 million people, or 42% of the population, in sub-Saharan Africa.

The vegetation of the Mediterranean coastal region of Egypt is considered to be one of its major natural resources. Its proper utilization plays a key role in the sound of this region which is known to have enjoyed prosperity during the Graeco-Romoan times [3] [4] .

The narrow areas of the fertile lands of the River Nile Region (Nile Valley and Nile Delta), though occupy less than 5% of Egypt’s area they represent the backbone of the cultivation of the traditional crops that are fully utilizing the freshwater of the River Nile. Thus, there is no surplus of Nile water that can be used for any agro-industrial and social activities in Egypt’s deserts which should depend mainly upon their own natural resources of water to grow and establish non-conventional crops having low water requirements to be selected from the desert plants.

Therefore, the communities in Egypt have a long history in directed towards the utilization of the renewable resources of the non-cultivated areas to produce more food for people, forage for animals and industrialization our local raw materials [5] .

In addition to some earlier works on this topic [6] -[11] , recent publications [1] [12] -[15] have investigated the nutritional value of non-traditional wild plants and utilization of these plants as renewable resources for raw materials.

The plants under investigation are found in Egypt in abundance and there for I think to throw the light on the importance of these plants. We are in Egypt caring about finding a source of plants as fodder because we have an abroad plan for animal breeding. The purpose of this study is to conduct a preliminary assessment of the nutritive value of some common wild plants as non-traditional fodder producing plants. These plants are Calligonum polygonoides, Cakile maritima, Senecio glaucus and Zygophullum album.

2. Materials and Methods

2.1. Study Area

The middle sections of Mediterranean coastal desert of Egypt (the Deltaic Coast) runs from Abu Qir to Port Said for about 180 km, has a width from sea landward (southwards) for about 15 km (Figure 1). It is characterized by 3 shallow natural lakes: Manzala (in the east), Burullus (in the middle) and Idku (in the west). These lakes receive the main bulks of the drainage waters from the Nile Delta lands [16] .

Figure 1. Map of the Nile Delta region showing different localities (^*) of the studied area.

The climate of the whole stretch of the Mediterranean coastal desert is generally, less arid than the remaining southern parts of Egypt. According to the Bioclimatic Map of (UNESCO/FAO, 1963), it belongs to the subdesertic warm climate. In the deltaic Mediterranean section annual rainfall ranges between 160 mm in Rosetta and 102.3 mm in Damietta.

Maximum air temperature in both coastal cities ranges between 18.1˚C to 30.2˚C and 18.4˚C to 31.3˚C whereas minimum temperatures range between 11.3˚C to 23˚C and 8.2˚C to 21.5˚C in January and July respectively. Relative humidity does not vary widely between the two cities being 76% and 74% and 83% and 75% in January and July respectively [16] .

2.2. Samples

Nutritional analyses (moisture, protein, fat, fibre, ash and minerals) were conducted using shoot system of fourwild plants (Calligonum polygonoides, Cakile maritima, Senecio glaucus and Zygophullum album) growing naturally in Deltaic Mediterranean coast, Egypt.

Approximately, 2 kg of shoot material were selected from several plants of each variety. Local floristic keys were used for determining the species. Data on different species and vernacular name have been sourced from publications on the flora of Egypt [17] see (Table 1).

The plants were processed on the same day of harvest. Died, yellow leaves were removed from the stems, shoot system washed thoroughly with distilled water until no foreign material remained, and air dried at room temperature for 2 h. Finally, the dried samples were stored in a dark cupboard in capped bottles in desiccators and used within 1 month after harvesting.

2.3. Chemical Analysis

Moisture content, total ash content, fat and fiber were analyzed by the methods described in AOAC [18] . The total nitrogen was determined by the Kjeldahl method [19] . Total protein was calculated by multiplying the total nitrogen by the factor 6.25 [18] . Glucose was determined based on the method of Feteris [20] . Sucrose was determined according to [21] . Na⁺ and K⁺ were determined photometrically whereas Ca²⁺, Mg²⁺, Fe²⁺, Mn²⁺, Zn²⁺, Cu²⁺, Cd²⁺, Co²⁺, Ni²⁺ and Pb²⁺ were estimated by using Atomic Absorption Spectrometer (A Perkin-Elemer, Model 2380, U.S.A.). The elements were expressed as mg/g according to Allen et al. [22] . The calculation of energy (kcal/100 g fresh weight) was carried out using the Atwater system as described by the World Health Organization (1985) by multiplying the values obtained for protein, carbohydrates and fat by 4.00, 3.75 and 9.00, respectively; the results are expressed in kcal. The total digestible nutrient was estimated according to the equation described by Abu El-Naga and EL-Shazly [23] .

2.4. Determination of Antioxidant Activity Scavenging Activity of DPPH Radical

In order to measure antioxidant activity, DPPH free radical scavenging assay was used according to a procedure

Table 1

Species, habitat, duration, life form, chorotype and occurrence of studied wild plants naturally growing in Deltaic Mediterranean coast, Egypt. Th, Therophytes; Nph, Nanophanerophytes; Ch, Chamaephytes; ME, Mediterranean; IR-TR, Irano-Turanian; ER-SR, Euro-Siberian; SA-SI, Saharo-Sindian

Scientific name	Vernacular name	Family	Duration	Life form	Chorotype	Habitat	Occurrence
Cakile maritima Scop.	Sea rocket	Brassicaceae	Annual	Th	ME+ER-SR	Mediterranean seashores and Nile Delta	Abundant
Calligonum polygonoides L.	Arta plant	Polygonaceae	Perennial	Nph	SA-SI+IR-TR	Sand formations	Common
Senecio glaucus L.	Almorrar	Asteraceae	Annual	Th	ME+SA-SI+IR-TR	Egyptian deserts and Mediterranean coastal strip	Very abundant
Zygophyllum album L.	Rotreit	Zygophyllaceae	Perennial	Ch	ME+SA-SI	Mediterranean seashores, Sinai, Oases and Nile Delta (around the lakes)	Common

based on Lim and Quah [24] as follows: two milliliters of 0.15 mM DPPH was added to 1 ml of various plant extracts in different concentrations. A control was prepared by adding 2 ml of DPPH to 1 ml solvent (methanol 50 %). The contents of the tubes were mixed and allowed to stand for 30 min., the absorbance was measured at 517 nm. The antioxidant activity was expressed as:

2.5. Statistical Analyses

One-way analysis of variance (ANOVA) was applied to assess the significance of variation in the nutritive values with equal replication using the COSTAT program.

3. Results

3.1. Proximate Composition

The composition of the four wild studied species is presented (Table 2). Shoot of Cakile maritima and Calligonum polygonoides yielded the highest energy levels of 353.74 kcal/100 g and 343.71 kcal/100 g, respectively.

Other parts of wild species have significant energy values ranging from 322.49 to 333.60 kcal/100 g. The total digestible nutrient ringing from 46.66 (shoot of Cakile maritima) to 57.13 (root of Senecio glaucus). All samples contained between 5.21% and 11.63% moisture. There was a slight variation in the fat content of the investigated wild species, ranging from 0.75% (root of Zygophyllum album) to 1.93% (shoot of Cakile maritima).

Shoot of Cakile maritima (19.63%), Calligonum polygonoides (18.94%) and Zygophyllum album (17.96%) stand out as being good sources of protein. Ash content ranged from 5.99% in shoot of Zygophyllum album to 9.27% in shoot of Senecio glaucus .The root of Cakile maritima and Zygophyllum album are good source of fibre (9.26% and 10.11%, respectively), while the lowest quantity of fibre was found in the shoot of Cakile maritima (5.52%).

Glucose and sucrose contentsattained the lowest value (1.56 and 2.31 mg/g, respectively) in root of Zygophyllum album, while the highest value (5.01 and 7.40 mg/g, respectively) in shoot of Cakile maritima. There was a slightly variation in carbohydrate of the studied species, ranging from 66.94 mg/g for Calligonum polygonoides to 78.77 for Zygophyllum album.

3.2. Mineral Levels

The concentrations of the macrominerals (N, P, K⁺, Ca⁺⁺, Mg⁺⁺ and Na⁺) estimated in the four studied species

Table 2

Moisture and ash contents as well as the constituents of studied plants. Car, carbohydrates; TDN, Total digestable nutrients

Nutrients	Plant part	Moisture	Ash		Fiber	Fat	Protein	Glucose	Sucrose	Calculated parameters Car.
		%						mg/g		%	Energy kcal/100g	TDN
Calligonum polygonoides	Shoot	5.21 ± 0.38		6.12 ± 0.07	7.02 ± 0.03	1.88 ± 0.02	18.94 ± 0.24	4.58 ± 0.3	5.89 ± 0.3	66.94	343.71	47.20
	Root	6.01 ± 0.80		7.22 ± 0.02	7.79 ± 0.05	1.00 ± 0.02	10.25 ± 0.13	2.34 ± 0.12	3.58 ± 0.31	74.31	328.66	54.14
Cakile maritima	Shoot	9.45 ± 0.70		4.84 ± 0.06	5.52 ± 0.02	1.93 ± 0.02	19.63 ± 0.22	5.01 ± 0.25	7.61 ± 0.24	68.76	353.74	46.66
	Root	7.81 ± 1.08		7.69 ± 0.94	9.26 ± 0.05	0.90 ± 0.05	8.56 ± 0.02	1.85 ± 0.13	2.87 ± 0.06	75.16	324.19	55.44
Senecio glaucus	Shoot	8.31 ± 1.15		9.27 ± 0.30	6.90 ± 0.44	1.40 ± 0.04	9.94 ± 0.13	2.94 ± 0.02	5.98 ± 0.05	72.86	322.49	54.70
	Root	6.22 ± 0.7		7.71 ± 0.25	6.35 ± 0.32	1.29 ± 0.19	5.60 ± 0.22	3.97 ± 0.03	6.91 ± 0.02	77.03	323.17	57.13
Zygophyllum album	Shoot	11.63 ± 1.40		5.99 ± 0.04	6.32 ± 0.09	1.72 ± 0.02	17.96 ± 0.27	3.86 ± 0.21	4.55 ± 0.13	68.34	333.60	48.03
	Root	8.24 ± 0.88		6.49 ± 3.01	10.11 ± 0.05	0.75 ± 0.03	7.50 ± 0.13	1.56 ± 0.06	2.31 ± 0.05	78.77	332.14	56.32
LSD0.05		3.59		3.34	0.12	0.08	0.6	0.91	0.90	4.11	5.62	5.59

are presented in Table 3. The nitrogen and potassium content of the species analyzed in this study showed remarkably high amounts in shoot than root. The highest value (31.40 and 28.10 mg/g dry weight, respectively) was observed in shoot of Cakile maritima, while the lowest value (8.95and 8.21mg/g dry weight, respectively) was recorded for Senecio glaucus roots. The phosphorus content of the wild species varied greatly and rangedfrom 1.67 mg/g (root of Zygophyllum album) to 4.02 mg/ g (shoot of Cakile maritima).

Sodium concentration showed little variation and ranged from 2.20 mg/g (shoot of Calligonum polygonoides) to 5.33 mg/g (root of Senecio glaucus), while calcium and magnesium concentration showed remarkably high amounts in root than shoot. The highest value (28.60 and 11.17 mg/g dry weight, respectively) was estimated in root of Zygophyllum album, while the lowest value (10.91 and 5.89 mg/g dry weight, respectively) was attained in shoot of Senecio glaucus.

On the other hand, the highest concentrations of the microminerals were recorded for F²⁺, Mn²⁺ and Zn²⁺ (4.521, 2.753 and 1.827 mg/100 g dry weight) in shoot of Senecio glaucus and Cu²⁺ (3.377 mg/100g dry weight) in shoot of Calligonum polygonoides. While, the lowest value (0.990, 0.510, 0.424 and 0.166 mg/100 g dry weight, respectively) were attained in root of Zygophyllum album. The highest concentrations of Co²⁺ and Ni²⁺ (0.148 and 0.291 mg/g dry weight, respectively) were recorded in root of Senecio glaucus, while Pb²⁺ and Cd²⁺ (0.866 and 0.018 mg/100 g dry weight, respectively) were observed in Senecio glaucus shoots. The lowest concen trations (0.062, 0.128, 0.349 and 0.007 mg/100g dry weight) were recorded in Cakile maritima shoots (Table 4).

3.3. Antioxidant

By increasing the plant extract concentration there was a corresponding continuous increase in scavenging activity (Figures 2-5). In case of Senecio glaucus shoot and root extract the increase was up to 500 µg/ml where the scavenging activity was 24.22% and 22.97%, respectively. For Calligonum polygonoides the scavenging activity was 16.96% for root and 12.08% for shoot. However, in case of Zygophyllum album the scavenging activity was 16.36% for shoot and 11.20% for root. Finally, in case of Cakile maritima attained high scavenging activity in shoot extract (14.78%), while in root extract attained 12.32%.

4. Discussion

The natural vegetation of Egypt’s deserts comprises considerable number of palatable species and/or species rich with their nutritive values that may be considered reliable natural resources for fodder production [25] .

Phytochemical analysis of the wild plants indicated that almost all plants are important and there is a need to develop techniques for cultivation and processing of such plants for future use. In our present study, most com-

Table 3

Analysis of macrominerals in plants under study

Species	Plant part	Nitrogen	Phosphorus	Sodium	Potassium	Calcium	Magnesium
		N	P	Na	K	Ca	Mg
		mg/g dry weight
Calligonum polygonoides	Shoot	30.30 ± 0.38	3.79 ± 0.02	2.20 ± 0.21	27.37 ± 0.17	18.87 ± 0.12	7.13 ± 0.20
	Root	16.40 ± 0.20	2.02 ± 0.03	3.07 ± 0.23	13.67 ± 2.19	25.80 ± 0.32	9.67 ± 0.41
Cakile maritima	Shoot	31.40 ± 0.35	4.02 ± 0.04	2.63 ± 0.13	28.10 ± 0.12	19.80 ± 0.35	7.77 ± 0.07
	Root	13.70 ± 0.32	1.77 ± 0.03	3.33 ± 0.23	10.90 ± 0.23	26.53 ± 0.23	10.90 ± 0.23
Senecio glaucus	Shoot	15.44 ± 0.21	3.10 ± 0.02	3.41 ± 0.12	11.94 ± 0.15	10.91 ± 0.32	5.89 ± 0.23
	Root	8.95 ± 0.24	1.81 ± 0.03	5.33 ± 0.24	8.21 ± 0.32	12.90 ± 0.15	9.03 ± 0.12
Zygophyllum album	Shoot	28.73 ± 0.43	3.52 ± 0.05	2.67 ± 0.24	25.20 ± 0.17	20.83 ± 0.27	8.67 ± 0.09
	Root	12.00 ± 0.21	1.67 ± 0.03	4.07 ± 0.12	9.87 ± 0.35	28.60 ± 0.32	11.17 ± 0.15
LSD0.05		0.6	0.4	0.61	2.41	1.1	0.9

Table 4

Analysis of microminerals in plants under study

Species	Plant part	Iron	Copper	Manganese	Zinc	Cobalt	Nickel	Lead	Cadmium
		Fe	Cu	Mn	Zn	Co	Ni	Pb	Cd
		mg/100g dry weight
Calligonum polygonoides	Shoot	2.370 ± 0.03	3.377 ± 2.46	1.100 ± 0.03	0.840 ± 0.02	0.079 ± 0.001	0.154 ± 0.006	0.387 ± 0.004	0.009 ± 0.000
	Root	1.525 ± 0.03	0.237 ± 0.01	0.660 ± 0.02	0.556 ± 0.02	0.089 ± 0.001	0.180 ± 0.004	0.480 ± 0.002	0.010 ± 0.000
Cakilemaritima	Shoot	2.395 ± 0.02	0.402 ± 0.01	1.310 ± 0.03	0.733 ± 0.09	0.062 ± 0.001	0.128 ± 0.004	0.349 ± 0.003	0.007 ± 0.000
	Root	1.125 ± 0.01	0.194 ± 0.01	0.600 ± 0.02	0.483 ± 0.01	0.094 ± 0.003	0.206 ± 0.004	0.568 ± 0.005	0.012 ± 0.000
Senecioglaucus	Shoot	4.521 ± 0.05	0.498 ± 0.01	2.753 ± 0.06	1.827 ± 0.02	0.126 ± 0.000	0.257 ± 0.000	0.866 ± 0.000	0.018 ± 0.009
	Root	2.482 ± 0.04	0.263 ± 0.03	1.195 ± 0.03	1.267 ± 0.03	0.148 ± 0.000	0.291 ± 0.000	0.778 ± 0.000	0.015 ± 0.017
Zygophyllum album	Shoot	2.220 ± 0.02	0.396 ± 0.01	1.220 ± 0.03	0.811 ± 0.01	0.085 ± 0.003	0.154 ± 0.008	0.409 ± 0.008	0.008 ± 0.000
	Root	0.990 ± 0.02	0.166 ± 0.01	0.510 ± 0.02	0.424 ± 0.02	0.111 ± 0.003	0.221 ± 0.005	0.576 ± 0.004	0.013 ± 0.000
LSD0.05		0.14	0.11	0.17	0.11	0.013	0.02	0.08	0.64

Figure 2

Scavenging activity of methanolic extract of Calligonum polygonoides

Figure 3

Scavenging activity of methanolic extract of Cakile maritima

monly used plants were evaluated for the nutritional values with special references to fiber, protein and fat content, reducing sugars, carbohydrates and energy.

Figure 4

Scavenging activity of methanolic extract of Senecio glaucus

Figure 5

Scavenging activity of methanolic extract of Zygophyllum album

Proteins and carbohydrates are the building blocks of the body and so are required in high amounts in regular diet. Similarly, lipids are the integral part in the cellular membranes and are required for various body functions [26] .

Presence of high crude fiber in food material is reported to decrease dry matter digestibility in animals. The high crude fiber content therefore provides a good indication of nutritive value of the feed material [27] .

The protein content of the investigated wild species ranged from 5.90% to 19.63% and fat in between 0.75% to 1.93%. Van Wyk [28] and Odhav et al. [29] reported the protein and fat values for Chenopodium album (4.4% and 0.3%), Amaranthus hybridus (3.5% and 0.3%) and Galinsoga parviflora (3.29% and 2.06%). The protein and fat contents of the wild plants in this study are relatively comparable to that obtained by El-Amier and Ejgholi [15] on Atriplex halimus (14.79 and 1.05%), Limonium pruinosum (12.38% and 0.92%) and Limoniastrum monopetalum (14.15% and 1.16%), but lower than those reported by Zahran and El-Amier [1] on Bassia indica (9.25 and 2.9%), Arthrocnemum macrostachyum (5.34% and 1.2%) and Halocnemum strobilaceum (9.44% and 2.13%) and Stanaćev et al. [30] on Medicago sativa and Trifolium alexandrinum.

The total digestible nutrient (TDN) is only an approximate measure of the food energy available to animals after digestion losses have been deduced [31] . Abd-El-Razik et al. [32] reported that the annual average of TDN value was 75% DM. In the studied species, the annual average of TDN is about 52.45% these results are comparable to those of clover (56%), barley (64%) and corn (68%) as reported by Soliman and EL-Shazly [33] .

The findings of the moisture, ash and fiber content of most of the wild plants analysed in this study conform the previous published literature for wild species of Atriplex halimus, Limonium pruinosum and Limoniastrum monopetalum [15] , on Bassia indica, Arthrocnemum macrostachyum and Halocnemum strobilaceum [1] , Atriplex halimus [34] , Medicago sativa [30] and Trifolium alexandrinum [35] .

Macro- and micro-minerals are very important in a required amount to achieve maximum health and production, as excess or lower amount is harmful in plants and animals [36] . Also, the results of the present study show that the concentrations of macro-elements (sodium, potassium and calcium) in the selected species are enough for the requirements of animals which reflects the findings of Mayland and Hankins [37] .

Generally, the four studied species contained concentrations of Na⁺, K⁺, Ca²⁺ and Mg²⁺, are in harmony with that those reported in Medicago sativa [38] and Trifolium alexandrinum [39] , but lower than those reported by Otal et al. [40] , Zahran and El Amier [1] on halophytic vegetation as well as Okon and James [41] on woody leafy vegetables (Heinsia crinata, Lasienthera africana and Pterocarpus mildbraedii). Nitrogen and phosphorus contents of the investigated wild species ranging from 9.43 - 31.40 mg/g dry wt. and 1.67 - 4.02 mg/g dry wt. in this study higher than those reported by Rathod et al. [2] on some selected wild edible plants (Ensete superbum, Oroxylum indicum, Smilax zeylanica, etc.) and Kalita et al. [42] on edible food plants (Solanum spirale and Cyathea spinulosa) as good source of mineral. Variations in the chemical composition of wild species, including the quantities of compounds are influenced by prevailing environmental conditions, the age of plants at harvest, which affects their genetic composition [29] [43] .

The results of the present work seem to be promise compared with the results reported previously by other investigates in the field of fodder and food plants. For example, the contents of protein and fat were19.63% and 1.93% which are higher than those reported by Zahran and El-Amier [1] on halophytic plants as fodder. Van Wyk [28] has reported the availability of some wild plants as food; however the reported protein and fat contents were lower than that in the present investigation.

5. Conclusions

Land use with shortage of fresh water in the deserts may limit agriculture and farming. Conventional crops need lots of fresh water and their cultivation in the deserts will, certainly, cause quick depletion of the ground water [1] .

It is worth to state that, according to the obtained results, the four species, namely: Calligonum polygonoides, Cakile maritima, Senecio glaucus (Psammophytes) and Zygophullum album (halophyte) naturally growing in the Deltaic Mediterranean coastal desert of Egypt are of high potentialities as fodder producing plants. They are rich with their nutritive values and their water requirements are low. Thus, mass production of their vegetative yields will certainly help to maintain reasonable quantities of raw materials for fodder industries.

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