<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd">
<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article">
 <front>
  <journal-meta>
   <journal-id journal-id-type="publisher-id">
    ars
   </journal-id>
   <journal-title-group>
    <journal-title>
     Advances in Remote Sensing
    </journal-title>
   </journal-title-group>
   <issn pub-type="epub">
    2169-267X
   </issn>
   <issn publication-format="print">
    2169-2688
   </issn>
   <publisher>
    <publisher-name>
     Scientific Research Publishing
    </publisher-name>
   </publisher>
  </journal-meta>
  <article-meta>
   <article-id pub-id-type="doi">
    10.4236/ars.2025.142006
   </article-id>
   <article-id pub-id-type="publisher-id">
    ars-143706
   </article-id>
   <article-categories>
    <subj-group subj-group-type="heading">
     <subject>
      Articles
     </subject>
    </subj-group>
    <subj-group subj-group-type="Discipline-v2">
     <subject>
      Computer Science 
     </subject>
     <subject>
       Communications
     </subject>
    </subj-group>
   </article-categories>
   <title-group>
    Quantifying Temporal Distortions of Artificial UAV Crop Canopy Temperature Measurements
   </title-group>
   <contrib-group>
    <contrib contrib-type="author" xlink:type="simple">
     <name name-style="western">
      <surname>
       Timothy S.
      </surname>
      <given-names>
       Goebel
      </given-names>
     </name>
    </contrib>
    <contrib contrib-type="author" xlink:type="simple">
     <name name-style="western">
      <surname>
       James R.
      </surname>
      <given-names>
       Mahan
      </given-names>
     </name>
    </contrib>
    <contrib contrib-type="author" xlink:type="simple">
     <name name-style="western">
      <surname>
       Paxton
      </surname>
      <given-names>
       Payton
      </given-names>
     </name>
    </contrib>
    <contrib contrib-type="author" xlink:type="simple">
     <name name-style="western">
      <surname>
       Andrew
      </surname>
      <given-names>
       Young
      </given-names>
     </name>
    </contrib>
    <contrib contrib-type="author" xlink:type="simple">
     <name name-style="western">
      <surname>
       N. Ace
      </surname>
      <given-names>
       Pugh
      </given-names>
     </name>
    </contrib>
    <contrib contrib-type="author" xlink:type="simple">
     <name name-style="western">
      <surname>
       Zhanguo
      </surname>
      <given-names>
       Xin
      </given-names>
     </name>
    </contrib>
    <contrib contrib-type="author" xlink:type="simple">
     <name name-style="western">
      <surname>
       John E.
      </surname>
      <given-names>
       Stout
      </given-names>
     </name>
    </contrib>
    <contrib contrib-type="author" xlink:type="simple">
     <name name-style="western">
      <surname>
       Robert J.
      </surname>
      <given-names>
       Lascano
      </given-names>
     </name>
    </contrib>
   </contrib-group> 
   <aff id="affnull">
    <addr-line>
     aUSDA-ARS, Cropping Systems Research Laboratory, Lubbock, TX, USA
    </addr-line> 
   </aff> 
   <pub-date pub-type="epub">
    <day>
     30
    </day> 
    <month>
     06
    </month>
    <year>
     2025
    </year>
   </pub-date> 
   <volume>
    14
   </volume> 
   <issue>
    02
   </issue>
   <fpage>
    87
   </fpage>
   <lpage>
    102
   </lpage>
   <history>
    <date date-type="received">
     <day>
      21,
     </day>
     <month>
      May
     </month>
     <year>
      2025
     </year>
    </date>
    <date date-type="published">
     <day>
      27,
     </day>
     <month>
      May
     </month>
     <year>
      2025
     </year> 
    </date> 
    <date date-type="accepted">
     <day>
      27,
     </day>
     <month>
      June
     </month>
     <year>
      2025
     </year> 
    </date>
   </history>
   <permissions>
    <copyright-statement>
     © Copyright 2014 by authors and Scientific Research Publishing Inc. 
    </copyright-statement>
    <copyright-year>
     2014
    </copyright-year>
    <license>
     <license-p>
      This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/
     </license-p>
    </license>
   </permissions>
   <abstract>
    The crop canopy temperature (T
    <sub>c</sub>) measured with fixed-infrared thermometers (IRT’s), provides high-resolution data but only measures small areas of the canopy. Conversely, thermal sensors mounted on UAVs measure T
    <sub>c</sub> over larger areas, overcoming this limitation. However, these measurements may introduce distortions of T
    <sub>c</sub>’s values due to the time of day when they are measured. We measured T
    <sub>c</sub> of a cotton crop over a growing season using fixed-IRTs and compared these measurements to the same data with an assumed time delay of 0.25 - 2 hours. This delay was used as a proxy of T
    <sub>c</sub> values measured with a UAV. The dataset consisted of 7 IRTs measuring 96 values/day over 67 days. Results showed that artificial UAV flight missions resulted in a thermal distortion related to the flight duration. This distortion was applied to detect differences of T
    <sub>c</sub> in seven irrigation treatments. The difference in T
    <sub>c</sub> from the UAV and fixed IRT was affected by the time of day, irrigation treatment, and UAV-flight duration. To identify irrigation treatments, the assumed UAV-T
    <sub>c</sub> produced up to 27% spurious treatment differences relative to the fixed-IRT. Distortion in UAV-T
    <sub>c</sub> was minimal for flights of &lt;15-minutes. Interpretation of UAV-T
    <sub>c</sub> data should consider this distortion.
   </abstract>
   <kwd-group> 
    <kwd>
     Fixed-Infrared Thermometer (Fixed-IRT)
    </kwd> 
    <kwd>
      Irrigation Treatment
    </kwd> 
    <kwd>
      Thermal Distortion
    </kwd>
   </kwd-group>
  </article-meta>
 </front>
 <body>
  <sec id="s1">
   <title>1. Introduction</title>
   <p>The use of plant temperature as an indicator of metabolic status has been used for more than 25 years <xref ref-type="bibr" rid="scirp.143706-1">
     [1]
    </xref>-<xref ref-type="bibr" rid="scirp.143706-6">
     [6]
    </xref>. Transpiration alters the temperature of the leaves <xref ref-type="bibr" rid="scirp.143706-1">
     [1]
    </xref> <xref ref-type="bibr" rid="scirp.143706-3">
     [3]
    </xref> and this temperature change can be measured continuously under a variety of conditions in a non-destructive and reproducible manner <xref ref-type="bibr" rid="scirp.143706-2">
     [2]
    </xref> <xref ref-type="bibr" rid="scirp.143706-6">
     [6]
    </xref>.</p>
   <p>In agricultural settings, the crop canopy temperature (T<sub>c</sub>) is often measured with infrared thermometers (IRT) placed among the plants in a field. These stationary IRT sensors are referred to herein as “fixed-IRTs”. While fixed-IRTs measure data with high temporal resolution, i.e., 15-minute data across the growing season, they are stationary and generally have a spatial resolution of &lt;0.5 m<sup>2</sup>. Given this limitation, there is an interest in increasing the spatial resolution by moving the sensor throughout the field either by walking with a handheld version of the instrument, attaching a sensor to a vehicle, or using a small Unmanned Aerial Vehicle (UAV). These platforms can provide the necessary spatial coverage and resolution; however, they are limited in their ability to collect repeated measurements, resulting in reduced temporal resolution <xref ref-type="bibr" rid="scirp.143706-7">
     [7]
    </xref>-<xref ref-type="bibr" rid="scirp.143706-9">
     [9]
    </xref>. Small unoccupied aircraft systems can be equipped with a variety of sensors including red, green, blue cameras (RGB), multispectral sensors, and thermal sensors <xref ref-type="bibr" rid="scirp.143706-10">
     [10]
    </xref>. Although many studies have used RGB and multispectral sensors, few studies have investigated best practices for the use of thermographic imagery collected by a UAV. Beyond the inherent difficulties involved in calibrating thermographic imagery, it is also crucial that parameters outside of the UAV or its payload be considered, e.g., the weather conditions and the altitude of the flight <xref ref-type="bibr" rid="scirp.143706-11">
     [11]
    </xref> <xref ref-type="bibr" rid="scirp.143706-12">
     [12]
    </xref>. Further temporal considerations, such as the time of day and differentials in temperature from the beginning of a flight mission to the end, are also important factors when designing thermal flight missions that have not been evaluated <xref ref-type="bibr" rid="scirp.143706-12">
     [12]
    </xref>. A small UAV equipped with thermal sensors offers researchers and producers the ability to monitor T<sub>c</sub> over many hectares at daily intervals <xref ref-type="bibr" rid="scirp.143706-13">
     [13]
    </xref>-<xref ref-type="bibr" rid="scirp.143706-16">
     [16]
    </xref>.</p>
   <p>With regard to irrigation, one must consider two important factors: when to irrigate and how much water to apply. The frequency of irrigation depends on the availability of irrigation-water and the amount to apply depends on the demand, a function of the environment and crop growth stage. Past research has addressed the different methods to measure and calculate crop evapotranspiration (ET) over the length of the growing season <xref ref-type="bibr" rid="scirp.143706-17">
     [17]
    </xref>-<xref ref-type="bibr" rid="scirp.143706-19">
     [19]
    </xref>. Examples of these measurements include field lysimeters <xref ref-type="bibr" rid="scirp.143706-20">
     [20]
    </xref> <xref ref-type="bibr" rid="scirp.143706-21">
     [21]
    </xref>, stem flow gauges <xref ref-type="bibr" rid="scirp.143706-22">
     [22]
    </xref>, water-balance by measuring soil water content as a function of depth and time, among other methods. A commonly used technique to calculate crop ET is the so-called “engineering method”, first proposed by Jensen <xref ref-type="bibr" rid="scirp.143706-23">
     [23]
    </xref> and discussed by Lascano <xref ref-type="bibr" rid="scirp.143706-24">
     [24]
    </xref>. This method provides an hourly or daily estimate of reference crop ET, for either a short or a tall crop, using the well-known Penman-Monteith equation and a crop-specific coefficient to calculate a corresponding crop ET <xref ref-type="bibr" rid="scirp.143706-25">
     [25]
    </xref>. Another well-known method is the Crop Water Stress Index (CWSI), used to calculate the relative transpiration rate of the plants at the time of measurement and is calculated from measurements of the T<sub>c</sub> and vapor pressure deficit <xref ref-type="bibr" rid="scirp.143706-26">
     [26]
    </xref> <xref ref-type="bibr" rid="scirp.143706-27">
     [27]
    </xref>. A variant of CWSI, called BIOTIC, uses T<sub>c</sub> and time thresholds to determine when to irrigate and how much water to apply <xref ref-type="bibr" rid="scirp.143706-28">
     [28]
    </xref>. Regardless of the method used, it is apparent that both measurements and calculations of crop ET require meteorological sensors that are expensive and require maintenance to manage the daily water requirement of a crop. However, this management scheme is not practical for large field applications. An alternative method is the use of a UAV equipped with a variety of sensors, i.e., RGB, multispectral and thermal, that can cover large fields. Furthermore, the cost of this technology continues to decline as smaller and more cost-effective hardware becomes available. For example, an orthoimage developed from a UAV flight can be assembled from hundreds of individual images in a manner that does not retain information regarding the time of measurement. The temperature data in an orthoimage is measured on a per pixel value and the assembly of the orthoimage uses software that does not provide any of the associated metadata (timestamp) of the assembled pixel. Without the timestamp of when the pixel was taken it is therefore difficult to determine the corresponding values of air temperature, humidity, wind speed and global irradiance that are needed to calculate values of crop ET, CWSI, etc. that change with time. In this paper we support the use of UAVs to measure the necessary data to calculate crop ET but suggest that some of the drawbacks need to be considered, which are often ignored when extracting data from thermal orthomosaics.</p>
   <p>Replacing fixed-IRTs with mobile platforms will reduce the number of measurements made per day. For example, using a single sensor and moving it in the field will require a large investment of time because the user must move between sampling locations and the elapsed time can range from seconds (UAV measurements) to minutes (ground measurements). In an agricultural setting, measurements of T<sub>c</sub> will be made in an environment where the T<sub>c</sub> is continuously changing. Given that the results from moving a sensor around a field is to evaluate the field spatially and not temporally, care must be taken to ensure that diurnal changes in T<sub>c</sub> do not artificially distort the spatial measurements. Given this limitation, it was of interest to evaluate the maximum amount of time between two T<sub>c</sub> measurements before the measurements can no longer be compared spatially due to temporal T<sub>c</sub> changes. To address this issue, the objectives of this study were to: 1) artificially change the T<sub>c</sub> occurring between the thermal measurements captured at different time intervals using a UAV-thermal platform as the basis for comparison in an agricultural setting, and 2) use data from an irrigation treatment experiment to artificially generate canopies with different crop water status from no irrigation to an application of 6 mm/d.</p>
  </sec><sec id="s2">
   <title>2. Materials and Methods</title>
   <sec id="s2_1">
    <title>2.1. Overview of Approach</title>
    <p>For this study, we used archived T<sub>c</sub> data from an irrigation experiment conducted in 2009 that were temporally distorted in silico and then compared to the original T<sub>c</sub> values. The data consisted of T<sub>c</sub> measurements recorded in 15-minute intervals collected from seven irrigation treatments (ranging from 0 to 6 mm/d) over the course of 67 days <xref ref-type="bibr" rid="scirp.143706-27">
      [27]
     </xref>. These original T<sub>c </sub>data were used to evaluate how often all seven treatments were distinguished from each other. Additionally, the data were offset by time (ranging from 15 to 120 min) to recreate the changes in T<sub>c</sub> that would occur over different UAV-thermal mission flight durations. In the context of this study, we introduced an artificial UAV-T<sub>c</sub> measurements as a proxy dataset of T<sub>c</sub> values to evaluate the temporal effect. The benefit of T<sub>c</sub> data in 15-minute intervals is that T<sub>c</sub> is determined with a high degree of precision over time. For this study, we assumed that this would approximate a UAV having imaged that small portion of the field where the sensor was located in similar 15-minute intervals. Therefore, it was possible to offset the time course of the T<sub>c</sub> data to artificially generate images collected using a UAV that occurred 15, 30, 45, 60, 90 and 120 minutes later. These data were then analyzed to examine the effect of flight duration on T<sub>c</sub> differences across the 7 irrigation treatments. In addition, this analysis allowed for the accurate quantification of temporal differences in crop water status. Using the T<sub>c </sub>data in this fashion allowed for the creation of a T<sub>c </sub>dataset to which deviations could be compared. For the purposes of this study, these data are referred to as “Standard T<sub>c</sub>”. These Standard T<sub>c</sub> data had high temporal resolutions which would have been difficult to obtain using a UAV-based thermal imaging system alone.</p>
   </sec>
   <sec id="s2_2">
    <title>2.2. Plot Characteristics</title>
    <p>The continuous data were collected in 2009 at the Texas Tech University Research farm in Lubbock, TX <xref ref-type="bibr" rid="scirp.143706-27">
      [27]
     </xref>. Cotton (DeltaPine 147RF, Monsanto, St. Louis, MO, USA) was planted on 15 May 2009, Day of Year (DOY 135). The soil of all sampled plots is classified as an Amarillo soil series (fine-loamy, mixed, superactive, thermic Aridic Paleustalfs). Agronomic practices were in accord with regional norms. Irrigated plots ~ 0.20 ha in size (4 rows × 10 m length) were watered with a sub-surface drip irrigation system with one irrigation line/row. Drip irrigation lines were placed 0.20 m below the planted rows, which were 1-m apart. Plots were irrigated daily beginning 53 days after planting for 67 days from DOY 188 to DOY 264 with the following amounts: 1, 2, 3, 4, 5, and 6 mm.</p>
   </sec>
   <sec id="s2_3">
    <title>2.3. Thermal Data Collection</title>
    <p>The changes in T<sub>c</sub> were measured with infrared thermometers (IRT) (SmartCrop™ Smartfield Inc., Lubbock, TX, USA) that were factory calibrated. One fixed-IRT was placed in each irrigation zone/irrigation treatment. The fixed-IRTs were positioned ~ 0.2 m above the plant canopy at a 60˚ oblique angle. Each fixed-IRT had a 1:1 view ratio with a 0.2 m height placement that corresponded to a 0.2-m radial view of the canopy, and the height and distance from the canopy were adjusted on a weekly basis. The measured changes in T<sub>c</sub> from each fixed-IRT were collected at 1-minute intervals, and values were recorded as 15-minute averages. This resulted in 96 T<sub>c</sub> measurements per day and per sensor across the 67-day study period from 11 July (DOY 192) 2009 to 15 September (DOY 258) 2009. In summary, the seasonal dataset for the seven fixed-IRTs consisted of 45,024 T<sub>c</sub> values.</p>
   </sec>
   <sec id="s2_4">
    <title>2.4. Artificial Generation of UAV-Based Temporal Offset</title>
    <p>The thermal UAV offset temperatures were calculated using the T<sub>c</sub> data. Thermal time separations were created by using the T<sub>c</sub> measurements in the subsequent 15-minute period as the offset reading. This was done for 15-, 30-, 45-, 60-, 90-, and 120-minute thermal separations on the irrigation treatments 0-6 mm (<xref ref-type="table" rid="table1">
      Table 1
     </xref>). This method resulted in Standard T<sub>c</sub> data and six UAV-thermal data sets of 15-, 30-, 45-, 60-, 90-, and 120-min flight durations.</p>
   </sec>
   <sec id="s2_5">
    <title>2.5. Treatment Separation Events</title>
    <p>In the dataset from <xref ref-type="bibr" rid="scirp.143706-27">
      [27]
     </xref>, there were seven irrigation treatments that were combined into six irrigation treatment pairs for comparisons of T<sub>c</sub> measurements over time. For each time point in the dataset, the irrigation treatment pairs consisted of T<sub>c</sub> values for two irrigation treatments that differed in daily irrigation amount by 1 mm. Each treatment pair consisted of one lower irrigation treatment and one higher irrigation treatment, e.g., 0 mm/d and 1 mm/d. The lower irrigation treatment tended to have a higher T<sub>c </sub>than the higher irrigation treatment. Differences in T<sub>c</sub> value among treatment pairs were identified in a two-step process, as follows:</p>
    <p>1) The difference (∆) in T<sub>c</sub> was calculated by subtracting the higher irrigation treatment from the lower irrigation treatment.</p>
    <p>2) The T<sub>c</sub> ∆s were then converted to a binary format, where 1 indicated that the absolute value of ∆ was &gt; than 0.5˚C (the thermal resolution of the IRT) and a 0 indicated that the absolute value of the ∆ was ≤0.5˚C.</p>
    <p>The resulting binary data were used throughout the rest of the analyses as a simple way to differentiate between two treatments that were thermally distinct from each other at any given point in time. Thermal separations &gt; 0.5˚C were considered a positive thermal separation; hereafter, referred to as treatment separation events (TSE). The TSEs were calculated for every time period during the 67-day study.</p>
    <table-wrap id="table1">
     <label>
      <xref ref-type="table" rid="table1">
       Table 1
      </xref></label>
     <caption>
      <title>
       <xref ref-type="bibr" rid="scirp.143706-"></xref>Table 1. Development of the artificial T<sub>c</sub> dataset for UAV-thermal mission length. All data were collected on 12 July 2009 (DOY 193) at various times (hh:mm) of day. Letters (A~D) denote data used to develop the artificial dataset for further analysis. The bold and colored numbers in the “0 mm” column represent the initial value at Time 15:00 as indicated for other columns.</title>
     </caption>
     <table class="MsoTableGrid custom-table" border="0" cellspacing="0" cellpadding="0"> 
      <tr> 
       <td class="custom-bottom-td acenter" width="9.15%"><p style="text-align:center">Time</p></td> 
       <td class="custom-bottom-td acenter" width="14.52%"><p style="text-align:center">Duration (min)</p></td> 
       <td class="custom-bottom-td acenter" width="8.42%"><p style="text-align:center">0 mm</p></td> 
       <td class="custom-bottom-td acenter" width="15.54%"><p style="text-align:center">0 mm - 30 min</p></td> 
       <td class="custom-bottom-td acenter" width="16.98%"><p style="text-align:center">0 mm - 60 min (B)</p></td> 
       <td class="custom-bottom-td acenter" width="17.14%"><p style="text-align:center">0 mm - 90 min (C)</p></td> 
       <td class="custom-bottom-td acenter" width="18.24%"><p style="text-align:center">0 mm - 120 min (D)</p></td> 
      </tr> 
      <tr> 
       <td class="custom-top-td acenter" width="9.15%"><p style="text-align:center">15:00</p></td> 
       <td class="custom-top-td acenter" width="14.52%"><p style="text-align:center">0</p></td> 
       <td class="custom-top-td acenter" width="8.42%"><p style="text-align:center">40.94</p></td> 
       <td class="custom-top-td acenter" width="15.54%"><p style="text-align:center">40.37</p></td> 
       <td class="custom-top-td acenter" width="16.98%"><p style="text-align:center">40.13</p></td> 
       <td class="custom-top-td acenter" width="17.14%"><p style="text-align:center">38.46</p></td> 
       <td class="custom-top-td acenter" width="18.24%"><p style="text-align:center">38.41</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="9.15%"><p style="text-align:center">15:15</p></td> 
       <td class="acenter" width="14.52%"><p style="text-align:center">15</p></td> 
       <td class="acenter" width="8.42%"><p style="text-align:center">41.29</p></td> 
       <td class="acenter" width="15.54%"><p style="text-align:center">40.14</p></td> 
       <td class="acenter" width="16.98%"><p style="text-align:center">39.77</p></td> 
       <td class="acenter" width="17.14%"><p style="text-align:center">38.38</p></td> 
       <td class="acenter" width="18.24%"><p style="text-align:center">37.92</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="9.15%"><p style="text-align:center">15:30</p></td> 
       <td class="acenter" width="14.52%"><p style="text-align:center">30</p></td> 
       <td class="acenter" width="8.42%"><p style="text-align:center">40.37</p></td> 
       <td class="acenter" width="15.54%"><p style="text-align:center">40.13</p></td> 
       <td class="acenter" width="16.98%"><p style="text-align:center">38.46</p></td> 
       <td class="acenter" width="17.14%"><p style="text-align:center">38.41</p></td> 
       <td class="acenter" width="18.24%"><p style="text-align:center">36.89</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="9.15%"><p style="text-align:center">15:45</p></td> 
       <td class="acenter" width="14.52%"><p style="text-align:center">45</p></td> 
       <td class="acenter" width="8.42%"><p style="text-align:center">40.14</p></td> 
       <td class="acenter" width="15.54%"><p style="text-align:center">39.77</p></td> 
       <td class="acenter" width="16.98%"><p style="text-align:center">38.38</p></td> 
       <td class="acenter" width="17.14%"><p style="text-align:center">37.92</p></td> 
       <td class="acenter" width="18.24%"><p style="text-align:center">36.11</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="9.15%"><p style="text-align:center">16:00</p></td> 
       <td class="acenter" width="14.52%"><p style="text-align:center">60</p></td> 
       <td class="acenter" width="8.42%"><p style="text-align:center">40.13</p></td> 
       <td class="acenter" width="15.54%"><p style="text-align:center">38.46</p></td> 
       <td class="acenter" width="16.98%"><p style="text-align:center">38.41</p></td> 
       <td class="acenter" width="17.14%"><p style="text-align:center">36.89</p></td> 
       <td class="acenter" width="18.24%"><p style="text-align:center">34.53</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="9.15%"><p style="text-align:center">16:15</p></td> 
       <td class="acenter" width="14.52%"><p style="text-align:center">75</p></td> 
       <td class="acenter" width="8.42%"><p style="text-align:center">39.77</p></td> 
       <td class="acenter" width="15.54%"><p style="text-align:center">38.38</p></td> 
       <td class="acenter" width="16.98%"><p style="text-align:center">37.92</p></td> 
       <td class="acenter" width="17.14%"><p style="text-align:center">36.11</p></td> 
       <td class="acenter" width="18.24%"><p style="text-align:center">34.07</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="9.15%"><p style="text-align:center">16:30</p></td> 
       <td class="acenter" width="14.52%"><p style="text-align:center">90</p></td> 
       <td class="acenter" width="8.42%"><p style="text-align:center">38.46</p></td> 
       <td class="acenter" width="15.54%"><p style="text-align:center">38.41</p></td> 
       <td class="acenter" width="16.98%"><p style="text-align:center">36.89</p></td> 
       <td class="acenter" width="17.14%"><p style="text-align:center">34.53</p></td> 
       <td class="acenter" width="18.24%"><p style="text-align:center">34.02</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="9.15%"><p style="text-align:center">16:45</p></td> 
       <td class="acenter" width="14.52%"><p style="text-align:center">105</p></td> 
       <td class="acenter" width="8.42%"><p style="text-align:center">38.38</p></td> 
       <td class="acenter" width="15.54%"><p style="text-align:center">37.92</p></td> 
       <td class="acenter" width="16.98%"><p style="text-align:center">36.11</p></td> 
       <td class="acenter" width="17.14%"><p style="text-align:center">34.07</p></td> 
       <td class="acenter" width="18.24%"><p style="text-align:center">33.62</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="9.15%"><p style="text-align:center">17:00</p></td> 
       <td class="acenter" width="14.52%"><p style="text-align:center">120</p></td> 
       <td class="acenter" width="8.42%"><p style="text-align:center">38.41</p></td> 
       <td class="acenter" width="15.54%"><p style="text-align:center">36.89</p></td> 
       <td class="acenter" width="16.98%"><p style="text-align:center">34.53</p></td> 
       <td class="acenter" width="17.14%"><p style="text-align:center">34.02</p></td> 
       <td class="acenter" width="18.24%"><p style="text-align:center">32.64</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="9.15%"><p style="text-align:center">17:15</p></td> 
       <td class="acenter" width="14.52%"><p style="text-align:center">135</p></td> 
       <td class="acenter" width="8.42%"><p style="text-align:center">37.92</p></td> 
       <td class="acenter" width="15.54%"><p style="text-align:center">36.11</p></td> 
       <td class="acenter" width="16.98%"><p style="text-align:center">34.07</p></td> 
       <td class="acenter" width="17.14%"><p style="text-align:center">33.62</p></td> 
       <td class="acenter" width="18.24%"><p style="text-align:center">32.58</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="9.15%"><p style="text-align:center">17:30</p></td> 
       <td class="acenter" width="14.52%"><p style="text-align:center">150</p></td> 
       <td class="acenter" width="8.42%"><p style="text-align:center">36.89</p></td> 
       <td class="acenter" width="15.54%"><p style="text-align:center">34.53</p></td> 
       <td class="acenter" width="16.98%"><p style="text-align:center">34.02</p></td> 
       <td class="acenter" width="17.14%"><p style="text-align:center">32.64</p></td> 
       <td class="acenter" width="18.24%"><p style="text-align:center">32.14</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="9.15%"><p style="text-align:center">17:45</p></td> 
       <td class="acenter" width="14.52%"><p style="text-align:center">165</p></td> 
       <td class="acenter" width="8.42%"><p style="text-align:center">36.11</p></td> 
       <td class="acenter" width="15.54%"><p style="text-align:center">34.07</p></td> 
       <td class="acenter" width="16.98%"><p style="text-align:center">33.62</p></td> 
       <td class="acenter" width="17.14%"><p style="text-align:center">32.58</p></td> 
       <td class="acenter" width="18.24%"><p style="text-align:center">31.99</p></td> 
      </tr> 
     </table>
    </table-wrap>
   </sec>
  </sec><sec id="s3">
   <title>3. Results</title>
   <p>The dataset used for analysis consisted of 45,024 values of measured T<sub>c</sub> from seven fixed-IRT’s in seven irrigation treatments over a 67-day study period. The measured T<sub>c </sub>data were used to generate an additional 270,144 artificial T<sub>c</sub> data that approximated typical UAV-thermal missions of varying duration. This set of measured and artificial T<sub>c</sub> values, 315,168 measurements in total, were used in the analysis of the effect of differential irrigation on cotton T<sub>c</sub> (<xref ref-type="fig" rid="fig1">
     Figure 1
    </xref>).</p>
   <sec id="s3_1">
    <title>3.1. Reduction from 15-Minute to 1-Hour T<sub>c</sub> Values during Daylight Hours</title>
    <p>The goal of the study was to use T<sub>c</sub> comparisons over time to identify irrigation responses in fixed-IRT data and to compare these results with those that could be obtained using the artificial UAV-thermal approach. A single UAV-thermal mission conducted per day during daylight hours was considered a reasonable goal for a field study. To recreate this daily mission, the dataset were reduced to hourly values for subsequent analyses. The primary approach was to perform the analysis in two sections:</p>
    <p>1) in the first section, fixed-IRT data were analyzed regarding the ability for each to identify the irrigation treatment effects; and</p>
    <p>2) the second section applies the same analysis to T<sub>c</sub> data that were temporally offset to provide UAV-thermal recreations.</p>
    <fig id="fig1" position="float">
     <label>Figure 1</label>
     <caption>
      <title>Figure 1. Canopy temperature during the 67-day study period measured every 15 minutes for the seven irrigation treatments. (A) The full-scale y-axis is 15˚C to 40˚C, (B) Canopy temperature is scaled to accentuate irrigation effects, and full-scale y-axis is 27˚C to 40˚C.</title>
     </caption>
     <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2630421-rId14.jpeg?20250630034651" />
    </fig>
   </sec>
   <sec id="s3_2">
    <title>3.2. Analysis of Measured T<sub>c</sub> from Fixed-IRT Sensors</title>
    <p>The goal of this study was to detect differences among irrigation treatments based on T<sub>c </sub>values. Seven irrigation treatments were grouped into six treatment pairs that represent 1 mm differences in daily irrigation amount. For example, the 0 mm/d and 1 mm/d irrigation treatments were paired to produce the 0 - 1 mm treatment pair. This produced the following irrigation treatment pairs; 0 - 1 mm, 1 - 2 mm, 2 - 3 mm, 3 - 4 mm, 4 - 5 mm, and 5 - 6 mm.</p>
    <p>For each treatment pair at each interval, T<sub>c</sub> data were compared to determine if they should be considered thermally separated. A separation for an irrigation treatment pair was defined as a T<sub>c</sub> difference &gt; 0.5˚C. For each comparison a TSE was assigned a value of 1 otherwise 0 was assigned according to the criteria described above.</p>
    <p>With six treatment pairs over the 67-day study period, there were 402 treatment comparisons/hour in each day. The results of six treatment comparisons for each hour between 08:00 and 20:00 (13 hour/d) for the 67-day period are shown (<xref ref-type="fig" rid="fig2">
      Figure 2
     </xref>). For each day, at each hour, there are six treatment comparisons that could produce a maximum of six TSE for a total of 5226 treatment pair comparisons (6 treatment pairs × 13 hourly periods, × 67 days) (<xref ref-type="fig" rid="fig2">
      Figure 2
     </xref>).</p>
    <fig id="fig2" position="float">
     <label>Figure 2</label>
     <caption>
      <title>Figure 2. The hourly pattern of TSEs over 67 days for each day. There are six treatment pairs at each hourly measurement. Each TSE appears as a vertical mark. Panel A shows 67 days for 13 hours, and Panel B shows two weeks for 2 hours.</title>
     </caption>
     <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2630421-rId15.jpeg?20250630034653" />
    </fig>
    <p>The pattern of TSE varied based upon the day and time of the measurement (<xref ref-type="fig" rid="fig2">
      Figure 2
     </xref>). The number of positive TSE across the daytime period decreased in the hours close to dawn and dusk, with the highest density of positive TSE occurring from 11:00 to 18:00 hours. Examined across the entirety of the study, there was some seasonality and weather influence present (<xref ref-type="fig" rid="fig2">
      Figure 2
     </xref>). There was a higher density of TSE at the beginning of the study in July 2009 as well as in the middle of August 2009 (<xref ref-type="fig" rid="fig2">
      Figure 2
     </xref>). The pattern of TSEs for each irrigation pair at 18:00 across the entire 67-day study period shows a general trend of decreasing TSEs with increasing irrigation volumes suggesting that irrigation treatments are more difficult to resolve at higher irrigation volumes (<xref ref-type="fig" rid="fig3">
      Figure 3
     </xref>). The number of TSEs for each treatment pair illustrates the decrease in TSEs with increasing daily irrigation volumes (<xref ref-type="fig" rid="fig4">
      Figure 4
     </xref>).</p>
    <fig id="fig3" position="float">
     <label>Figure 3</label>
     <caption>
      <title>Figure 3. The 67-day pattern of TSEs at 18:00 hours separated by irrigation-treatment pair.</title>
     </caption>
     <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2630421-rId16.jpeg?20250630034653" />
    </fig>
    <fig id="fig4" position="float">
     <label>Figure 4</label>
     <caption>
      <title>Figure 4. Number of TSEs for each irrigation treatment pair at 18:00 hours for 67-days of the study. The treatments differ by 1 mm/day of irrigation.</title>
     </caption>
     <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2630421-rId17.jpeg?20250630034653" />
    </fig>
    <p>The UAV-thermal distortion alters both the number of TSE observed and the T<sub>c</sub> values associated with a given TSE. Treatment separation events were analyzed to reveal the occurrence of spurious TSE that were artifacts of the artificial UAV-thermal method (<xref ref-type="fig" rid="fig5">
      Figure 5
     </xref>). Spurious TSEs were identified by comparing the pattern of TSEs in the fixed-IRT data with the TSE pattern in the artificial UAV-thermal data. Two types of TSE errors were identified: i) TSE deletions, where a TSE in the fixed-IRT data was not present in a UAV-thermal pattern, and ii) TSE additions, where a TSE in the artificial UAV-thermal data is not present in the fixed-IRT data. For the purposes of this study, TSEs that were added were designated as Type I errors and TSEs that were deleted were designated as Type II errors. The extremes were used to demonstrate the identification of TSE errors (<xref ref-type="fig" rid="fig5">
      Figure 5
     </xref>). The same analysis was applied across the collection of six treatment pairs for seven flight durations over 67 days at 18:00 hours.</p>
    <p>In total, there were 2814 treatment pair comparisons resulting in 2161 TSE (<xref ref-type="table" rid="table2">
      Table 2
     </xref>). Of the 2161 TSE, 52 were Type II errors (2.4%) and 533 which were Type I errors (24.7%), resulting in 1680 true TSEs, i.e., present in both the fixed-IRT and sUAS-thermal data. In the fixed-IRT (0 minute) data there are 240 total TSEs; thus, TSEs more than 240 are a result of the distortion associated with flight mission durations. In addition, the relative number of Type I errors increased as irrigation levels increased. As the irrigation amount increased, true TSEs decreased, and Type I errors increased (<xref ref-type="fig" rid="fig5">
      Figure 5
     </xref>). Evaluating daytime hours (8:00 - 20:00) across the 67-day period for the Standard T<sub>c</sub> data as well as all artificial data clearly indicated a false reduction in TSEs with increasing flight time in the morning hours and a false increase in TSEs in the afternoon (<xref ref-type="fig" rid="fig6">
      Figure 6
     </xref>). Notably, the identification of the spurious TSEs was only possible because of the Standard T<sub>c</sub> data that could be used for comparison, which would not be available in data collected solely with a UAV-thermal sensor.</p>
    <fig id="fig5" position="float">
     <label>Figure 5</label>
     <caption>
      <title>Figure 5. Graphical representation of the TSE errors throughout the 67-day period. The upper panel shows the 56 TSEs from the fixed-IRT, the middle panel shows 66 TSEs from a 120-minute artificial UAV thermal, and lower panel shows ten false positives TSEs unique to the 0 - 1 mm 120-minute data.</title>
     </caption>
     <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2630421-rId18.jpeg?20250630034653" />
    </fig>
    <table-wrap id="table2">
     <label>
      <xref ref-type="table" rid="table2">
       Table 2
      </xref></label>
     <caption>
      <title>
       <xref ref-type="bibr" rid="scirp.143706-"></xref>Table 2. Summary of the number of TSE for each UAV-thermal flight time and irrigation treatment pairs with a summary of Type I and Type II errors.</title>
     </caption>
     <table class="MsoTableGrid custom-table" border="0" cellspacing="0" cellpadding="0"> 
      <tr> 
       <td class="custom-bottom-td acenter" width="20.77%"><p style="text-align:center">Irrigation Pairs</p></td> 
       <td class="custom-bottom-td acenter" width="11.91%"><p style="text-align:center">Fixed-IRT</p></td> 
       <td class="custom-bottom-td acenter" width="9.62%"><p style="text-align:center">15 min</p></td> 
       <td class="custom-bottom-td acenter" width="9.62%"><p style="text-align:center">30 min</p></td> 
       <td class="custom-bottom-td acenter" width="9.62%"><p style="text-align:center">45 min</p></td> 
       <td class="custom-bottom-td acenter" width="9.62%"><p style="text-align:center">60 min</p></td> 
       <td class="custom-bottom-td acenter" width="9.62%"><p style="text-align:center">90 min</p></td> 
       <td class="custom-bottom-td acenter" width="9.62%"><p style="text-align:center">120 min</p></td> 
       <td class="custom-bottom-td acenter" width="9.62%"><p style="text-align:center">Totals</p></td> 
      </tr> 
      <tr> 
       <td class="custom-top-td acenter" width="20.77%"><p style="text-align:center">0 - 1</p></td> 
       <td class="custom-top-td acenter" width="11.91%"><p style="text-align:center">56</p></td> 
       <td class="custom-top-td acenter" width="9.62%"><p style="text-align:center">59</p></td> 
       <td class="custom-top-td acenter" width="9.62%"><p style="text-align:center">63</p></td> 
       <td class="custom-top-td acenter" width="9.62%"><p style="text-align:center">64</p></td> 
       <td class="custom-top-td acenter" width="9.62%"><p style="text-align:center">64</p></td> 
       <td class="custom-top-td acenter" width="9.62%"><p style="text-align:center">66</p></td> 
       <td class="custom-top-td acenter" width="9.62%"><p style="text-align:center">66</p></td> 
       <td class="custom-top-td acenter" width="9.62%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="20.77%"><p style="text-align:center">1 - 2</p></td> 
       <td class="acenter" width="11.91%"><p style="text-align:center">46</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">51</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">59</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">60</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">64</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">65</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">65</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="20.77%"><p style="text-align:center">2 - 3</p></td> 
       <td class="acenter" width="11.91%"><p style="text-align:center">54</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">58</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">62</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">63</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">63</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">65</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">65</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="20.77%"><p style="text-align:center">3 - 4</p></td> 
       <td class="acenter" width="11.91%"><p style="text-align:center">43</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">45</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">57</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">57</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">60</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">61</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">62</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="20.77%"><p style="text-align:center">4 - 5</p></td> 
       <td class="acenter" width="11.91%"><p style="text-align:center">33</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">40</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">48</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">55</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">52</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">48</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">51</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="20.77%"><p style="text-align:center">5 - 6</p></td> 
       <td class="acenter" width="11.91%"><p style="text-align:center">8</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">8</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">16</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">27</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">34</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">37</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">41</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="20.77%"><p style="text-align:center"># Possible Comparisons</p></td> 
       <td class="acenter" width="11.91%"><p style="text-align:center">402</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">402</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">402</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">402</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">402</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">402</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">402</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">2814</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="20.77%"><p style="text-align:center">Total TSE</p></td> 
       <td class="acenter" width="11.91%"><p style="text-align:center">240</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">261</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">305</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">326</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">337</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">342</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">350</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">2161</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="20.77%"><p style="text-align:center"># Type I Errors</p></td> 
       <td class="acenter" width="11.91%"><p style="text-align:center">N/A*</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">34</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">69</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">93</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">104</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">112</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">121</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">533</p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="20.77%"><p style="text-align:center"># Type II errors</p></td> 
       <td class="acenter" width="11.91%"><p style="text-align:center">N/A*</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">13</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">4</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">7</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">7</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">10</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">11</p></td> 
       <td class="acenter" width="9.62%"><p style="text-align:center">52</p></td> 
      </tr> 
     </table>
    </table-wrap>
    <p>*by definition, there are no Type I errors.</p>
    <fig id="fig6" position="float">
     <label>Figure 6</label>
     <caption>
      <title>Figure 6. Full panel of TSE’s from 8:00 to 20:00 hours across 67-days, including all thermal distortions for all irrigation treatments and UAV-thermal flight duration.</title>
     </caption>
     <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2630421-rId19.jpeg?20250630034653" />
    </fig>
    <p>The effect of intermittent cloud cover during a thermal UAV flight was an additional point of concern in terms of contributing to Type I and Type II errors. <xref ref-type="fig" rid="fig7">
      Figure 7
     </xref> depicts the presence of TSEs as vertical bars for each treatment pair across each hour from 8:00 - 20:00. At 13:00, shortwave solar irradiance was reduced to 568 W/m<sup>2</sup> and there were only two Type I errors in the lower irrigation treatment comparisons, however, there were nine Type II errors in the higher irrigation treatment comparisons. At 14:00 (solar noon at 13:50) there was a reduction in net solar irradiance suggesting that clouds interfered with solar transmission. During this period with clouds reducing the net solar irradiance there were no TSEs present in any of the irrigation treatments; however, the Fixed-IRT showed there should be thirty Type II errors. At 16:00 the solar irradiance dipped again; however, there were no errors generated in any of the treatment comparisons. <xref ref-type="fig" rid="fig7">
      Figure 7
     </xref> suggests that the presence of intermittent cloud cover can create thermal artifacts in complex patterns that are difficult to identify.</p>
    <fig id="fig7" position="float">
     <label>Figure 7</label>
     <caption>
      <title>Figure 7. Effect of intermittent clouds on hourly TSEs for one day. The right panel is separated by treatment comparisons from 0 - 1 mm to 5 - 6 mm irrigation. Vertical bars denote a positive TSE, and red bars represent TSEs from the control (no shift in timestamp). The left side is the net solar radiation for the hour showing intermittent decreases due to clouds.</title>
     </caption>
     <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2630421-rId20.jpeg?20250630034653" />
    </fig>
   </sec>
   <sec id="s3_3">
    <title>3.3. Mitigation of UAV-Based Thermal Distortion</title>
    <p>The data presented in <xref ref-type="table" rid="table2">
      Table 2
     </xref> suggests a relationship between the flight duration, measurement area and thermal distortion that can be described for a representative UAV-thermal mission (<xref ref-type="fig" rid="fig8">
      Figure 8
     </xref>). This artificial AV uses a flight coverage of 0.1 acres/minute (405 m<sup>2</sup>/minute) at a flight height of 40 m <xref ref-type="bibr" rid="scirp.143706-29">
      [29]
     </xref>. The dashed line indicated the relationship between flight duration and measurement area. The thermal distortion associated with the UAV-thermal method is shown for each flight duration. Depending on what T<sub>c</sub> distortion would be considered acceptable, a potential thermal UAV flight time could be restricted to as little as 30 minutes (0.5˚C).</p>
   </sec>
  </sec><sec id="s4">
   <title>4. Discussion</title>
   <p>The purpose of this study was to compare the use of fixed-IRTs and UAV thermal sensors to detect differences among irrigation treatments in a cotton crop. The use of fixed-IRT sensors to detect differences in crop stress has been used in the past <xref ref-type="bibr" rid="scirp.143706-2">
     [2]
    </xref> <xref ref-type="bibr" rid="scirp.143706-3">
     [3]
    </xref>. The primary limitation of the fixed-IRT method is the reduced spatial footprint of the sensor. A UAV-thermal system has a much larger spatial footprint than a fixed-IRT sensor. A thermal artifact inherent in widely used UAV thermal approaches was explored in a previous study <xref ref-type="bibr" rid="scirp.143706-30">
     [30]
    </xref>. The TSE Type I error rate of 25% suggests there are potential complications that may arise when using UAV-based thermal methods. Regardless of the time of day or date that a flight mission is conducted, it appears likely that thermal distortion associated with the UAV method will be present.</p>
   <fig id="fig8" position="float">
    <label>Figure 8</label>
    <caption>
     <title>Figure 8. Ground area (ha) imaged in a UAV-thermal mission at a rate of 0.1 ha/minute as a function of the duration of the mission, which is shown by the dashed line. The colored lines represent the maximum thermal distortion in a UAV-thermal mission derived from temporal distortion of fixed-IRT data. Each line represents a different irrigation level.</title>
    </caption>
    <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2630421-rId21.jpeg?20250630034654" />
   </fig>
   <p>In this study, fixed-IRT T<sub>c</sub> data were used to create artificially time-shifted data from 15 minutes to 60 minutes and to explore how these time shifts change the resulting comparisons of irrigation treatments if it assumed that a time shift is not present. One of the potential drawbacks of this approach is that it does not provide a means to analyze the temporal distortion in UAV-thermal data. Thus, it is reasonable to postulate its existence and estimate its magnitude. It is not feasible to determine anything definitive about any specific data that are collected with a UAV-thermal sensor from the results presented herein. To the best of the authors’ knowledge, there are no methods for identifying or correcting for these errors using UAV-derived thermal images alone.</p>
   <p>Given that there is the potential for thermal distortion in UAV thermal T<sub>c</sub> measurements and that the distortion is difficult to detect and quantify, efforts to reduce the distortion could prove useful. The data show relationships among the flight duration, measurement area, and thermal distortion for representative UAV-thermal missions (<xref ref-type="fig" rid="fig8">
     Figure 8
    </xref>). These data demonstrate that the magnitude of the thermal anomaly increases with flight duration, a trend that is evident across the range of irrigation treatments; however, the magnitude of the thermal anomaly decreases with increasing irrigation volume. The thermal distortions associated with the artificial UAV-thermal methods indicate that the identification of irrigation treatment differences with UAV-derived thermal data may be limited compared to fixed-IRT methods. As the number of measurements decreased, the ability to resolve irrigation treatment differences was negatively affected. The smaller number of T<sub>c </sub>comparisons from a longer period between measurements would be compounded by the thermal distortion associated with UAV-thermal system that creates spurious differences among treatments.</p>
   <p>There are several ways to reduce the effects of UAV-thermal distortion. Since the T<sub>c </sub>is constantly changing during the period of measurement, the flight duration is the only parameter that can be controlled. Reduced flight duration is the most direct means to reduce UAV-thermal distortion. While decreasing the flight duration will reduce the thermal distortion, it will also reduce the area that can be measured (see <xref ref-type="fig" rid="fig8">
     Figure 8
    </xref>). In small plot studies the reduction of plot size can be accomplished but as the number of entities to measure increases, UAV-thermal imaging will become more complex. Increasing the imaging height will increase the area/image but may reduce the thermal and spatial resolution. Plot layout design with the temporal artifact in mind should be considered. The coincidence of increased image area and increased UAV-thermal distortion is potentially complex in large field studies. Field variability, e.g., soil type, irrigation efficiency, is often mitigated through replication. Replication increases plot area that in turn requires a larger study area. It is possible that under certain conditions the increased resolution achieved through replication would be offset by increased error in the thermal measurements. In such an instance, the potential improvements associated with increased reps should be considered due to the associated distortion of the thermal data resulting from increase study area. Finally, the inclusion of control plots with known thermal properties might prove useful though such mitigation is beyond the scope of this paper.</p>
   <p>One of the most important considerations of this study was to determine the accuracy of thermal orthomosaic derived from imagery collected by a UAV. The ability to assemble individual UAV-thermal images into orthomosaic representations of T<sub>c</sub> within a study area is a potential strength of UAV-thermal methods. Composite images are created by spatially aligning individual images into a mosaic image. Orthomosaic field representations from UAVs have proven useful in phenotyping crop characteristics such as plant density, ground cover, flowers, canopy height, and a host of others. However, T<sub>c</sub> changes continuously over short time periods; thus, while images from a UAV-thermal system can be spatially composited, a temporal thermal distortion is inherent in the resulting orthomosaic representations.</p>
   <p>While use of UAV-derived thermal data on orthomosaic representations has inherent limitations, T<sub>c </sub>data that are extracted from individual UAV images are likely useful in the detection of water use differences. The drawback of such imaging is that the spatial coverage is limited by the field of view of the thermal sensor and the altitude of the UAV when an image is collected. The balance between spatial coverage and thermal fidelity of UAV-thermal methods should be informed by knowledge of the thermal distortion that may occur. The data presented in this study should be sufficient to demonstrate UAV-thermal distortion. Users should be aware of these limitations when collecting thermographic data using UAV.</p>
  </sec><sec id="s5">
   <title>5. Conclusions</title>
   <p>Based on comparisons of fixed-IRT and artificial UAV-thermal data presented here, several key conclusions are drawn. A UAV-thermal sensor distorts T<sub>c</sub> values relative to those collected with a fixed-IRT. Additionally, the number of measurements that can be made using a UAV-thermal drone is limited compared to that of a fixed-IRT system, and the ability to differentiate T<sub>c</sub> related to irrigation treatments is reduced. The combination of thermal distortion and reduced measurement frequency alters the number and pattern of irrigation treatment differences that can be detected using T<sub>c</sub>. The temporal distortion can result in spurious treatment differences that diminish the utility of T<sub>c</sub> as a tool for assessing differences in water deficit effects. While improving the spatial resolution, a UAV platform creates multiple sources of error for T<sub>c</sub> measurements due to temporal distortion. While 0.5˚C was chosen as the threshold for these examples, other values that are either lower or higher might also be used to evaluate the effect of cutoff filters on treatment separations. In summary, the results from our analysis using artificially generated UAV thermal data demonstrated that implementing thermal UAV must be accompanied by a method to correct for the temporal distortion.</p>
  </sec><sec id="s6">
   <title>Declarations</title>
   <p>Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendations or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer.</p>
  </sec>
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