Assessment of the State of <i>Onobrychis tavernierfolia</i> Stocks ex Boiss.(Fabaceae) Cenopopulations Southwestern Kyzylkum(Uzbekistan)

In the period 2019-2021, two coenotic populations of O. tavernierfolia were studied in different ecological and phytocenotic conditions of relic mountain Kuldzhuktau. Very rare species of the Kyzylkum. It is spread at the Kyzylkum: the Kuldzhuktau, near Ayak-Guzhumdy and Churuk (Bukhara region). Inhabits stony and gypseous slopes. There are three small populations can be found. Limiting factors: degradation of habitat as a result of overgrazing. Measures of protection: not protected. The article is devoted to the assessment of the current state of coenotic populations of the rarest southwestern Kyzylkum (Kuldzhuktau) Onobrychis tavernierfolia. It was found that the studied coenopopulations of O. tavernierfolia are normal and complete.

• Onobrychis Tavernierfolia
• Cenopopulation
• Kyzylkum
• Assessment
• Rare Plant
• Population Signs

1. Introduction

Currently, population analysis methods are a reliable way to diagnose the conditions of populations of different plant species in natural and artificial communities. In addition, this set of methods provides the researcher with a departure from subjective visual assessments of the state of plants and plant communities by applying accurate quantitative accounting that reflects the internal plasticity and variability of plants.

Genus Onobrychis Mill it belongs to the tribe Hedysareae, the subfamily Faboidea, which is part of one of the largest families of flowering plants—Fabaceae, order Fabales. In the modern flora, the genus is represented by 170 species, which are widely distributed in the triangle of Anatolia-Iran-Caucasus.

The assessment of the state of the coenopopulation of O. tavernierfolia remains poorly understood. Onobrychis tavernierfolia Stocks ex Boiss. (Fabaceae)—a very rare and small annual plant of Kyzylkum desert (part Uzbekistan). One of the rare red book species with a disjunctive range from Central Kyzylkum to Central Iran, a remarkable ephemer—Onobrychis taevernierifolia is a new and very promising phytomeliorant. It is included in the “Red Book of the Republic of Uzbekistan” with the status 1 [1]. The purpose of this work is to study the state of the coenopopulations of Onobrychis taevernierifolia in southwestern Kyzylkumi (Kuldzhuktau).

2. Material and Methods

In the period 2019-2021, two coenotic populations of O. tavernierfolia were studied in different ecological-phytocenotic conditions of relic mountain Kuldzhuktau (Figure 1).

Geobotanical descriptions were performed according to the standard method at sites of 100 m [2].

According to the allocation characteristics of desert species, it was studied according to the method of M. V. Markov [3]. The relationship between the size of the reproductive force and the life form was studied. Eight features of the allocation characteristic were analyzed: the number of seeds per individual, the total mass of one individual, the mass of roots, the mass of the aboveground vegetative part, the mass of the generative part, the mass of one seed, the height of the

aboveground part, the length of the roots, and the vector spectrum was constructed. Additionally, the density of O. tavernierifolia individuals was studied.

3. Result and Discussion

The first cenopopulation was studied along the Churuk-Jangeldy highway in thean altitude of 417 m above sea level. Geographical coordinates of the cenopopulation: N 40044.816 E 063040.229. The soil of the described area is pebbly. The total projected grass cover is 22%. The botanical composition of the community is relatively rich and it consists of 25 species of vascular plants. The plant community is dominated by Salsolaarbuscula,Artemisia diffusa. The dominant species are Astragalusvillosissimus,Ammothamnus lehmanii,Ferula foetida,Tulipa sogdiana,Carex physodes, etc (Table 1).

The second cenopopulation is described 18 km north of the Kyzylkum desert station along the Shafirkan-Jangeldi road as part of the ephemeral-ephemeroid-sagebrush community. Geographical coordinates of the cenopopulation N 400 77.718 E 0630 58.843, height 373 m above sea level. The soil of the described area is gravelly. The total projected grass cover is 21%. The botanical composition of the communities consists of 34 species. The dominant community is Artemisia diffusa Krasch. ex Poljakov. Along with sagebrush, Astragalusammotrophus Bunge, Ferula foetida (Bunge) Regel, Cousiniahammadae Juz., Astragalusvillosisimus Bunge, etc. grow here (Table 1).

O. tavernierifolia Stocks ex Boiss.—annual squat plant, 3 - 7 cm tall. Almost stemless, the leaves on the upper side are covered with short pressed, and from below with long protruding white hairs, located on long (up to 5 cm) petioles ending in a large lobe-leaf obovate. Peduncles 2-3. 5 cm long. Brushes are loose, 3 - 7 flowered. Calyx 3-3. 5 cm long. The beans are wrinkly-cellular, about 1 cm long and wide. Blooms in May, bears fruit in June. Additionally, eight features of the allocation characteristic were analyzed: the number of seeds per individual, the total mass of one individual, the mass of roots, the mass of the aboveground vegetative part, the mass of the generative part, the mass of one seed, the height of the aboveground part, the length of the roots, and the vector spectrum was constructed.

The analysis of allocation polygrams showed that Onobrychis tavernierifolia has high indicators of the number of seeds (5 points) and the total mass of one individual (4 points), and less values are assigned to the height of the aboveground part of the individuals (2 points) and the length of the roots (2 points) (Figure 2).

Seed propagation is the most important stage in the life and development of plants, which ensures the preservation of the turnover (replacement) of generations and, thanks to passive movement, the settlement of populations. In this regard, seeds represent units of reproduction and settlement, means for experiencing unfavorable conditions, and are also a bank of genetic variants that are formed as a result of combinative variability. Seeds store information about the

Table 1

Species composition and abundance of the O. tavernierfolia community

№	Species	Life form	Projective coverplants, % (CP)
			CP 1	CP 2
1	Salsola arbuscula Pall.	Shrub	5	1
2	Astragalus villosissimus Bunge	Shrub	+	1
3	Ammothamnus lehmanii Bunge	Shrub	+	−
4	Artemisia diffusa Krasch.ex Poljakov	Semi-shrub	7	5
5	Acanthophyllum stenostegium Freyn	Semi-shrub	−	2
6	Calligonum zakirovii (Chalkoziev)	Shrub	+	−
7	Astragalus ammotrophus Bunge	Perennial	−	1
8	Cousinia hammadae Juz.	Perennial	−	+
9	Ixiolirion tataricum (Pall.) Schult. & Schult. fil	Perennial	−	+
10	Ferula foetida (Bunge) Regel	Perennial	2	2
11	Tulipa sogdiana Bunge	Perennial	4	−
12	Carex physodes Bieb.	Perennial	3	3
13	Peganumharmala L.	Perennial		+
14	Poa bulbosa L.	Perennial	+
15	Allium kyzylkumii Kamelin	Perennial	−	+
16	Eremurus korolkowii Regel	Perennial	−	+
17	Rhinopetalum karelinii Fisch.ex D. Don	Perennial	−	+
18	Tulipa lehmanniana Merkl	Perennial	+	+
19	Carex physodes Bieb.	Perennial	3	3
20	Amberboa turanica Iljin	Annual	+	+
21	Goldbachia laevigata (Bieb.) DC.	Annual	+	−
22	Hypecoum parviflorum Kar et. Kir	Annual	+	−
23	Nonea caspiсa (Willd.) G. Don	Annual	+	−
24	Roеmeria refracta (Stev.) DC	Annual	+	1
25	Onobrychistavernierifolia Stocks ex Boiss	Annual	+	+
26	Strigosella africana (L.) Boiss	Annual	+	+
27	Anisantha tectorum L.	Annual	+	+
28	Takhtajanianthapusilla (Pall.) Nazarova	Annual	−	+
29	Koelpinia linearis Pall.	Annual	−	+
30	Delphinium barbatum Bunge	Annual	−	+
32	Strigosella grandiflora (Bunge) Botsch	Annual	−	+
32	Ceratocephala testiculata (Crantz) Bess.	Annual	−	+
33	Alyssum desertorum Stapf.	Annual	−	+
34	Ziziphora tenior L.	Annual	−	+
35	Stipa hohenackeriana Trin. & Rupr	Annual	−	+
36	Bromus danthoniae Trin.	Annual	−	+
37	Amberboa turanica Iljin	Annual	−	+
38	Cuminium setifolium (Boiss.) Koso-Pol.	Annual	−	+
39	Ceratocephala falcata (L.) Pers.	Annual	+	2
40	Astragalus remanens Nabiev	Annual	+	−
41	Eremopyrum distans (C. Koch) Nevski	Annual	+	−
42	Lallimanthia royleana (Benth.) Bent	Annual	+	+
43	Holosteum polyganum C. Koch	Annual	+	−

principles of the system organization of the population and are able to recreate it under appropriate conditions). By Z. Shaniyazova [4], analysis of allocative signs in desert of ephemers are divided into four groups:

The first—with low values of the total biomass and seed production; the second—lowest value of the total biomass and average seed production; thirdly, medium or high values of the total biomass and high seed productivity, and a fourth having high or average values of the total biomass and low seed production (Figure 3; Table 2).

O. tavernierifolia belongs to the first group by the nature of allocation, characterized by low values of total biomass and low seed productivity (Table 3).

The density of ephemera populations can largely be determined by the amount of seed productivity, but it also depends on many parameters that characterize seeds as a special population group. For the permanent existence of desert annuals in the communities, the presence of a soil stock of seeds and the density of individuals is of particular importance.

At the sites, the soil stock of seeds was determined from a depth of 0 - 10 cm of soil. The maximum number of seeds of incoming seeds per year was noted in CP 2 (up to 9 pcs/1m²). On CP 1, this indicator is low (3 pcs/m²).

The density of O. tavernierifolia individuals at temporary sites in 2019 ranged from 0.9 to 2.5 individuals/m² and in 2020—0.1 - 3.3 m² (Figure 4). Similar variation in the density of individuals according to Z. Shaniyazova [4] is due to the fact that the seeds of desert ephemera do not have a period of endogenous rest or this period is very short and the possibility of germination is mainly determined by the external situation. According to the density of individuals in the transects desert ephemera are divided into 5 groups [4]:

1) The highest density—units of individuals/m², presence in the active state; 2) The highest density is a unit of individuals/m², the presence is almost constant; 3) The highest density-tens of individuals/m², the presence of episodic; 4) The highest density-tens of individuals/m², the presence of constant; 5) The highest density is hundreds of individuals/m², the presence is almost constant.

According to this classification, O. tavernierifolia belongs to the second group: the highest density is a unit of individuals/m², the presence is almost constant.

O. tavernierifolia is characterized by low seed productivity and accordingly with minimal seed entry into the soil and low density of individuals in coenopopulations. During the years of the study regardless of weather conditions, O. tavernierifolia individuals were constantly present in the transects (Figure 4).

Table 2

Population and organism parameters of O. tavernierifolia

№	Signs	Points
		I	II	III	IV	V
1	The number of seeds	5 - 5.4	5.5 - 5.8	5.9 - 6.2	6.3 - 6.6	6.7 - 7
2	The total mass of one individual. mg	0.37 - 0.45	0.46 - 0.54	0.55 - 0.63	0.63 - 0.72	0.73 - 0.85
3	The mass of the roots. mg	0.018 - 0.025	0.026 - 0.033	0.034 - 0.041	0.042 - 0.049	0.050 - 0.057
4	Mass vegetative part. mg	0.22 - 0.27	0.28 - 0.33	0.34 - 0.39	0.40 - 0.45	0.46 - 0.51
5	Mass generative part. g	0.05 - 0.116	0.007 - 0.183	0.184 - 0.25	0.26 - 0.326	0.327 - 0.384
6	The mass of one seed	0.017 - 0.023	0.024 - 0.031	0.032 - 0.039	0.040 - 0.045	0.046 - 0.055
7	The height of the aboveground part	2.80 - 2.93	2.94 - 3.01	3.02 - 3.09	3.10 - 3.17	3.18 - 3.25
8	The length of the roots. cm	8.3 - 8.8	8.9 - 9.5	9.6 - 10.2	10.3 - 10.8	10.9 - 11.3

Table 3

Seed productivity of O. tavernierifolia in transects

Years	Number of seeds per 1 individual
	min	max	Average	The error is average.
2019	3	7	4.7	0.37
2020	3	7	5.9	0.33

4. Conclusions

In practice, with the modern study of the species population, the main strategy for the protection of rare species is to protect their habitats, i.e., the creation of protected areas is one of the most important areas of human nature protection. However, the last 10 years in the world have seen a doubling in the number and increase in the area of specially protected natural areas of more than 30 million km². The results obtained from the evaluation of populations are used in carrying out monitoring studies for long years on rare plants. At the same time he used it as a material for local “Red books” [5] [6] [7].

The analysis of some indicators of O. tavernierifolia showed the following: having low values of total biomass and low seed productivity; the highest density is a unit of individuals/m² but the presence is almost constant. According to these characteristics O. tavernierifolia corresponds to the R-strategies and belongs to the group of tolerant species. The results obtained are used in plant protection.

Acknowledgements

The current research is done under the project PZ—20170919165 Inventory of rare and endangered species of vascular plants of Navoi and Bukhara regions.

Conflicts of Interest

The authors declare no conflicts of interest regarding the publication of this paper.

References

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